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n1 hypothalamic neuron cells  (Cedarlane)


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    Cedarlane n1 hypothalamic neuron cells
    a , b Effects of oleate or palmitate treatment (200 μM each for the indicated duration) on cellular NAD + contents in <t>N1</t> <t>hypothalamic</t> neuronal cells or primary cultured hypothalamic astrocytes ( n = 3 wells). c Schematic illustration depicting the various NAD + biosynthetic pathways. d Heatmaps showing the palmitate treatment (200 μM, 48 h)-induced alterations in expression levels of NAD + biosynthetic enzymes in hypothalamic neurons and astrocytes ( n = 3 wells). e , f Changes in nicotinamide phosphoribosyltransferase (NAMPT) protein expression and enzyme activity in hypothalamic astrocytes following palmitate treatment (200 μM, 48 h) ( n = 3 wells). g , h Effects of NAMPT inhibition using FK866 (500 nM, 48 h) or Nampt siRNA treatment on the palmitate-induced increase in the cellular NAD + levels of hypothalamic astrocytes ( n = 3 wells). i Palmitate treatment (200 μM) did not alter cellular NADH levels in primary hypothalamic astrocytes ( n = 3 wells). j Nampt fluorescence in situ hybridization (FISH) and GFAP dual staining showing the changes in Nampt mRNA expression in hypothalamic, cortical, and hippocampal astrocytes of 15-week-old C57 male mice fed either a CD or HFD for 4 weeks ( n = 120 astrocytes from 4 mice per group). Scale bars: 20 μm. arb. units: arbitrary unit. k qPCR analysis of Nampt expression in astrocytes isolated from different brain regions (hypothalamus, cortex, and hippocampus) of 15-week-old C57 male mice subjected to a CD or HFD for 4 weeks ( n = 3). l Palmitate treatment did not induce alterations in Nampt mRNA expression or cellular NAD + content in primary cortical astrocytes (Nampt, n = 6; NAD + , n = 3). One-way ANOVA followed by Fisher’s LSD test ( a , b , g , h- NAD + ) and two-sided unpaired t-test ( d – f , h- Nampt, i – l ). n.s.: not significant. Three independent replicates were performed for all studies and measurement were taken from distinct samples. Results are presented as the mea n ± SEM. Source data are provided as a Source Data file.
    N1 Hypothalamic Neuron Cells, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n1+hypothalamic+neuron+cells/pmc10920699-282-0-7?v=Cedarlane
    Average 93 stars, based on 8 article reviews
    n1 hypothalamic neuron cells - by Bioz Stars, 2026-07
    93/100 stars

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    1) Product Images from "Hypothalamic astrocyte NAD + salvage pathway mediates the coupling of dietary fat overconsumption in a mouse model of obesity"

    Article Title: Hypothalamic astrocyte NAD + salvage pathway mediates the coupling of dietary fat overconsumption in a mouse model of obesity

    Journal: Nature Communications

    doi: 10.1038/s41467-024-46009-0

    a , b Effects of oleate or palmitate treatment (200 μM each for the indicated duration) on cellular NAD + contents in N1 hypothalamic neuronal cells or primary cultured hypothalamic astrocytes ( n = 3 wells). c Schematic illustration depicting the various NAD + biosynthetic pathways. d Heatmaps showing the palmitate treatment (200 μM, 48 h)-induced alterations in expression levels of NAD + biosynthetic enzymes in hypothalamic neurons and astrocytes ( n = 3 wells). e , f Changes in nicotinamide phosphoribosyltransferase (NAMPT) protein expression and enzyme activity in hypothalamic astrocytes following palmitate treatment (200 μM, 48 h) ( n = 3 wells). g , h Effects of NAMPT inhibition using FK866 (500 nM, 48 h) or Nampt siRNA treatment on the palmitate-induced increase in the cellular NAD + levels of hypothalamic astrocytes ( n = 3 wells). i Palmitate treatment (200 μM) did not alter cellular NADH levels in primary hypothalamic astrocytes ( n = 3 wells). j Nampt fluorescence in situ hybridization (FISH) and GFAP dual staining showing the changes in Nampt mRNA expression in hypothalamic, cortical, and hippocampal astrocytes of 15-week-old C57 male mice fed either a CD or HFD for 4 weeks ( n = 120 astrocytes from 4 mice per group). Scale bars: 20 μm. arb. units: arbitrary unit. k qPCR analysis of Nampt expression in astrocytes isolated from different brain regions (hypothalamus, cortex, and hippocampus) of 15-week-old C57 male mice subjected to a CD or HFD for 4 weeks ( n = 3). l Palmitate treatment did not induce alterations in Nampt mRNA expression or cellular NAD + content in primary cortical astrocytes (Nampt, n = 6; NAD + , n = 3). One-way ANOVA followed by Fisher’s LSD test ( a , b , g , h- NAD + ) and two-sided unpaired t-test ( d – f , h- Nampt, i – l ). n.s.: not significant. Three independent replicates were performed for all studies and measurement were taken from distinct samples. Results are presented as the mea n ± SEM. Source data are provided as a Source Data file.
    Figure Legend Snippet: a , b Effects of oleate or palmitate treatment (200 μM each for the indicated duration) on cellular NAD + contents in N1 hypothalamic neuronal cells or primary cultured hypothalamic astrocytes ( n = 3 wells). c Schematic illustration depicting the various NAD + biosynthetic pathways. d Heatmaps showing the palmitate treatment (200 μM, 48 h)-induced alterations in expression levels of NAD + biosynthetic enzymes in hypothalamic neurons and astrocytes ( n = 3 wells). e , f Changes in nicotinamide phosphoribosyltransferase (NAMPT) protein expression and enzyme activity in hypothalamic astrocytes following palmitate treatment (200 μM, 48 h) ( n = 3 wells). g , h Effects of NAMPT inhibition using FK866 (500 nM, 48 h) or Nampt siRNA treatment on the palmitate-induced increase in the cellular NAD + levels of hypothalamic astrocytes ( n = 3 wells). i Palmitate treatment (200 μM) did not alter cellular NADH levels in primary hypothalamic astrocytes ( n = 3 wells). j Nampt fluorescence in situ hybridization (FISH) and GFAP dual staining showing the changes in Nampt mRNA expression in hypothalamic, cortical, and hippocampal astrocytes of 15-week-old C57 male mice fed either a CD or HFD for 4 weeks ( n = 120 astrocytes from 4 mice per group). Scale bars: 20 μm. arb. units: arbitrary unit. k qPCR analysis of Nampt expression in astrocytes isolated from different brain regions (hypothalamus, cortex, and hippocampus) of 15-week-old C57 male mice subjected to a CD or HFD for 4 weeks ( n = 3). l Palmitate treatment did not induce alterations in Nampt mRNA expression or cellular NAD + content in primary cortical astrocytes (Nampt, n = 6; NAD + , n = 3). One-way ANOVA followed by Fisher’s LSD test ( a , b , g , h- NAD + ) and two-sided unpaired t-test ( d – f , h- Nampt, i – l ). n.s.: not significant. Three independent replicates were performed for all studies and measurement were taken from distinct samples. Results are presented as the mea n ± SEM. Source data are provided as a Source Data file.

    Techniques Used: Cell Culture, Expressing, Activity Assay, Inhibition, Fluorescence, In Situ Hybridization, Staining, Isolation



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    Cedarlane n1 hypothalamic neuron cells
    a , b Effects of oleate or palmitate treatment (200 μM each for the indicated duration) on cellular NAD + contents in <t>N1</t> <t>hypothalamic</t> neuronal cells or primary cultured hypothalamic astrocytes ( n = 3 wells). c Schematic illustration depicting the various NAD + biosynthetic pathways. d Heatmaps showing the palmitate treatment (200 μM, 48 h)-induced alterations in expression levels of NAD + biosynthetic enzymes in hypothalamic neurons and astrocytes ( n = 3 wells). e , f Changes in nicotinamide phosphoribosyltransferase (NAMPT) protein expression and enzyme activity in hypothalamic astrocytes following palmitate treatment (200 μM, 48 h) ( n = 3 wells). g , h Effects of NAMPT inhibition using FK866 (500 nM, 48 h) or Nampt siRNA treatment on the palmitate-induced increase in the cellular NAD + levels of hypothalamic astrocytes ( n = 3 wells). i Palmitate treatment (200 μM) did not alter cellular NADH levels in primary hypothalamic astrocytes ( n = 3 wells). j Nampt fluorescence in situ hybridization (FISH) and GFAP dual staining showing the changes in Nampt mRNA expression in hypothalamic, cortical, and hippocampal astrocytes of 15-week-old C57 male mice fed either a CD or HFD for 4 weeks ( n = 120 astrocytes from 4 mice per group). Scale bars: 20 μm. arb. units: arbitrary unit. k qPCR analysis of Nampt expression in astrocytes isolated from different brain regions (hypothalamus, cortex, and hippocampus) of 15-week-old C57 male mice subjected to a CD or HFD for 4 weeks ( n = 3). l Palmitate treatment did not induce alterations in Nampt mRNA expression or cellular NAD + content in primary cortical astrocytes (Nampt, n = 6; NAD + , n = 3). One-way ANOVA followed by Fisher’s LSD test ( a , b , g , h- NAD + ) and two-sided unpaired t-test ( d – f , h- Nampt, i – l ). n.s.: not significant. Three independent replicates were performed for all studies and measurement were taken from distinct samples. Results are presented as the mea n ± SEM. Source data are provided as a Source Data file.
    N1 Hypothalamic Neuron Cells, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n1+hypothalamic+neuron+cells/pmc10920699-282-0-7?v=Cedarlane
    Average 93 stars, based on 1 article reviews
    n1 hypothalamic neuron cells - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier
    n1 murine hypothalamic neuron cells  (Cedarlane)
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    Cedarlane n1 murine hypothalamic neuron cells
    a , b Effects of oleate or palmitate treatment (200 μM each for the indicated duration) on cellular NAD + contents in <t>N1</t> <t>hypothalamic</t> neuronal cells or primary cultured hypothalamic astrocytes ( n = 3 wells). c Schematic illustration depicting the various NAD + biosynthetic pathways. d Heatmaps showing the palmitate treatment (200 μM, 48 h)-induced alterations in expression levels of NAD + biosynthetic enzymes in hypothalamic neurons and astrocytes ( n = 3 wells). e , f Changes in nicotinamide phosphoribosyltransferase (NAMPT) protein expression and enzyme activity in hypothalamic astrocytes following palmitate treatment (200 μM, 48 h) ( n = 3 wells). g , h Effects of NAMPT inhibition using FK866 (500 nM, 48 h) or Nampt siRNA treatment on the palmitate-induced increase in the cellular NAD + levels of hypothalamic astrocytes ( n = 3 wells). i Palmitate treatment (200 μM) did not alter cellular NADH levels in primary hypothalamic astrocytes ( n = 3 wells). j Nampt fluorescence in situ hybridization (FISH) and GFAP dual staining showing the changes in Nampt mRNA expression in hypothalamic, cortical, and hippocampal astrocytes of 15-week-old C57 male mice fed either a CD or HFD for 4 weeks ( n = 120 astrocytes from 4 mice per group). Scale bars: 20 μm. arb. units: arbitrary unit. k qPCR analysis of Nampt expression in astrocytes isolated from different brain regions (hypothalamus, cortex, and hippocampus) of 15-week-old C57 male mice subjected to a CD or HFD for 4 weeks ( n = 3). l Palmitate treatment did not induce alterations in Nampt mRNA expression or cellular NAD + content in primary cortical astrocytes (Nampt, n = 6; NAD + , n = 3). One-way ANOVA followed by Fisher’s LSD test ( a , b , g , h- NAD + ) and two-sided unpaired t-test ( d – f , h- Nampt, i – l ). n.s.: not significant. Three independent replicates were performed for all studies and measurement were taken from distinct samples. Results are presented as the mea n ± SEM. Source data are provided as a Source Data file.
    N1 Murine Hypothalamic Neuron Cells, supplied by Cedarlane, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    a , b Effects of oleate or palmitate treatment (200 μM each for the indicated duration) on cellular NAD + contents in N1 hypothalamic neuronal cells or primary cultured hypothalamic astrocytes ( n = 3 wells). c Schematic illustration depicting the various NAD + biosynthetic pathways. d Heatmaps showing the palmitate treatment (200 μM, 48 h)-induced alterations in expression levels of NAD + biosynthetic enzymes in hypothalamic neurons and astrocytes ( n = 3 wells). e , f Changes in nicotinamide phosphoribosyltransferase (NAMPT) protein expression and enzyme activity in hypothalamic astrocytes following palmitate treatment (200 μM, 48 h) ( n = 3 wells). g , h Effects of NAMPT inhibition using FK866 (500 nM, 48 h) or Nampt siRNA treatment on the palmitate-induced increase in the cellular NAD + levels of hypothalamic astrocytes ( n = 3 wells). i Palmitate treatment (200 μM) did not alter cellular NADH levels in primary hypothalamic astrocytes ( n = 3 wells). j Nampt fluorescence in situ hybridization (FISH) and GFAP dual staining showing the changes in Nampt mRNA expression in hypothalamic, cortical, and hippocampal astrocytes of 15-week-old C57 male mice fed either a CD or HFD for 4 weeks ( n = 120 astrocytes from 4 mice per group). Scale bars: 20 μm. arb. units: arbitrary unit. k qPCR analysis of Nampt expression in astrocytes isolated from different brain regions (hypothalamus, cortex, and hippocampus) of 15-week-old C57 male mice subjected to a CD or HFD for 4 weeks ( n = 3). l Palmitate treatment did not induce alterations in Nampt mRNA expression or cellular NAD + content in primary cortical astrocytes (Nampt, n = 6; NAD + , n = 3). One-way ANOVA followed by Fisher’s LSD test ( a , b , g , h- NAD + ) and two-sided unpaired t-test ( d – f , h- Nampt, i – l ). n.s.: not significant. Three independent replicates were performed for all studies and measurement were taken from distinct samples. Results are presented as the mea n ± SEM. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Hypothalamic astrocyte NAD + salvage pathway mediates the coupling of dietary fat overconsumption in a mouse model of obesity

    doi: 10.1038/s41467-024-46009-0

    Figure Lengend Snippet: a , b Effects of oleate or palmitate treatment (200 μM each for the indicated duration) on cellular NAD + contents in N1 hypothalamic neuronal cells or primary cultured hypothalamic astrocytes ( n = 3 wells). c Schematic illustration depicting the various NAD + biosynthetic pathways. d Heatmaps showing the palmitate treatment (200 μM, 48 h)-induced alterations in expression levels of NAD + biosynthetic enzymes in hypothalamic neurons and astrocytes ( n = 3 wells). e , f Changes in nicotinamide phosphoribosyltransferase (NAMPT) protein expression and enzyme activity in hypothalamic astrocytes following palmitate treatment (200 μM, 48 h) ( n = 3 wells). g , h Effects of NAMPT inhibition using FK866 (500 nM, 48 h) or Nampt siRNA treatment on the palmitate-induced increase in the cellular NAD + levels of hypothalamic astrocytes ( n = 3 wells). i Palmitate treatment (200 μM) did not alter cellular NADH levels in primary hypothalamic astrocytes ( n = 3 wells). j Nampt fluorescence in situ hybridization (FISH) and GFAP dual staining showing the changes in Nampt mRNA expression in hypothalamic, cortical, and hippocampal astrocytes of 15-week-old C57 male mice fed either a CD or HFD for 4 weeks ( n = 120 astrocytes from 4 mice per group). Scale bars: 20 μm. arb. units: arbitrary unit. k qPCR analysis of Nampt expression in astrocytes isolated from different brain regions (hypothalamus, cortex, and hippocampus) of 15-week-old C57 male mice subjected to a CD or HFD for 4 weeks ( n = 3). l Palmitate treatment did not induce alterations in Nampt mRNA expression or cellular NAD + content in primary cortical astrocytes (Nampt, n = 6; NAD + , n = 3). One-way ANOVA followed by Fisher’s LSD test ( a , b , g , h- NAD + ) and two-sided unpaired t-test ( d – f , h- Nampt, i – l ). n.s.: not significant. Three independent replicates were performed for all studies and measurement were taken from distinct samples. Results are presented as the mea n ± SEM. Source data are provided as a Source Data file.

    Article Snippet: N1 hypothalamic neuron cells were obtained from Cedarlane (CED-CLU101) and cultured in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin.

    Techniques: Cell Culture, Expressing, Activity Assay, Inhibition, Fluorescence, In Situ Hybridization, Staining, Isolation